Prolozone Therapy


What is Prolozone Therapy?

Prolozone Therapy is the injection of a mixture of numbing medicine, vitamins, homeopathics, and then ozone gas into muscles, joints, tendons and ligaments. Prolozone Therapy is a unique form of therapy that both heals and detoxifies at the same time. To understand what ozone therapy is, you need to know about ozone gas. Ozone is a molecule comprised of three oxygen atoms. The stratosphere has ozone gas. Lightning makes it when it splits pairs of oxygen atoms which then rush back together as a triplet. Your white blood cells make it. The Germans first developed ways of using ozone for medicinal purposes in the 1950's. Its use is now ubiquitous across Europe and is slowly catching on here in America.

What Health Problems is Prolozone Therapy Used for?

- Osteoarthritis or Rheumatoid arthritis in any joint it occurs (i.e. the neck, thoracic, lumbar, sacrum, shoulders, elbows, wrists, fingers, hips, knees, ankles, toes).

- Sprains or strains of any muscle or joint from overuse or from injury.

- Trigger Points or troublesome "knots" in the muscle.

- TMJ: It is hard to emphasize enough how many headaches or neck-pain cases have resolved just by applying Prolozone Therapy to the Temperal Mandibular Joint.

- Dental Pain or Infections - Prolozone applied to root canals or dental infections can clear up smoldering infections all together or at least stave off pain until your dentist can get you in. A lot of people have persistent dental infections that their dentists has kind of thrown up his hands about and are able to chew fine with dental Prolozone applied every few months when they feel it acting up.

- Cellulite: Ozone applied just below the skin can cause collagen regeneration and reverse cellulite.

How does Prolozone Therapy work?

Ozone balances the immune system, makes cells work more efficiently by making them metabolize with oxygen, improves blood circulation, increases the body's anti-inflammatory mechanisms, and increases the output of energy units in the cells.

One can refer to the articles sited below as a starting point for a more technical treatment of the mechanism of action of Prolozone Therapy.

What Happens When I Go For Prolozone Therapies?

Prolozone Therapy itself involves an injection of a mixture consisting of numbing medicine and other substances that tissue needs to heal like vitamins, anti-inflammatories and then a small amount of freshly made ozone gas. The tissues are very familiar with both ozone and oxygen gasses and readily absorb it. The doctor will then ask you to "test drive" the therapy to make sure it was delivered to exactly the right place. Patients are usually feel super right after the treatment. The following day some may experience muscle or joint soreness that is from toxins mobilizing. Pain relief from the ozone occurs in varying degrees depending on the person. Healing occurs from the one injection over the next three weeks.

How Many Sessions of Prolozone Therapy Will I Need?

Prolozone Therapy is usually done on a weekly basis for the first few injections and then spaced out based on your needs. It is important to realize that Prolozone Therapy is not a panacea or some kind of magic bullet. Although it is often an indispensable modality, it is only rarely effective by itself. In the great majority of cases it must be combined with an individualized program of other alternative and natural therapies, such as nutrition and detoxification. (Shallenberger)

Is Prolozone Safe?

Anything, including water and oxygen, is toxic if given in amounts that exceed the body's capacity to utilize it. Prolozone is found naturally in the body. The white cells make it as part of the immune response.Pure medical grade Prolozone, when it is used according to the established medical guidelines, has a safety record that is unparalleled.

Is There Evidence That Prolozone Works?

Here are some articles you can find on pubmed to get you started. Since this technology has been utilized for decades internationally, you can find many many articles and studies validating its use.

Saudi Med J. 2008 Apr;29(4):553-7.
Painkilling effect of ozone-oxygen injection on spine and joint osteoarthritis.
Al-Jaziri AA, Mahmoodi SM.

Departments of Surgery, Rashid Hospital, Dubai, United Arab Emirates.


To analyze the painkilling effect of ozone-oxygen injection on joint and spine osteoarthritis.

This prospective study was completed at the Ozone Clinic, Rashid Hospital, Dubai, United Arab Emirates on 220 mainly local patients 122 women, mean age 47.05 years; 98 men, mean age 52.8 years with radiographic documented spine or extremities osteoarthritis. The patients were treated over 3 years September 2002 to August 2005 by ozone-oxygen injection twice a week for at least 12 sessions. Using the 6 faces pain scale; the patients' pain was recorded at the beginning and at the 4th, 8th, and 12th sessions. They were followed for a mean of 8.48 months and their pain scale was recorded at that time too.

Comparison of the patients' 1st day pains with their 4th, 8th, and 12th sessions' pains showed a significant decrease 1st day to 4th session p=0.005, 1st day to 8th week p=0.005, 1st day to 12th session p=0.0043. Comparison of the 1st day pain with the final follow-up pain, which was around 10 months from the first treatment, showed a meaningful decrease of pain p=0.0048.

This study validates the painkilling effect of ozone-oxygen injection on osteoarthritis of the joints and spine. Its long term effect on pain advocates the likelihood of some histological changes as mechanism of its action.

PMID:18382798 [PubMed - indexed for MEDLINE] Neuroradiology. 2008 Sep;50(9):777-85. doi: 10.1007/s00234-008-0398-2. Epub 2008 May 16.

CT-guided ozone/steroid therapy for the treatment of degenerative spinal disease--effect of age, gender, disc pathology and multi-segmental changes.
Oder B, Loewe M, Reisegger M, Lang W, Ilias W, Thurnher SA.

Department of Radiology and Nuclear Medicine, Hospital Brothers of St. John of God, Vienna, Austria.


Oxygen-ozone nucleolysis (ONL) is a new, minimally invasive procedure for the treatment of discogenic low back pain with or without radicular symptoms. The aim of the present study was to determine associations between the morphology of the basic disease, patient-specific factors and the outcome of the treatment.

Six hundred and twelve patients not responding to conservative therapy were divided into five groups (disc bulging, disc herniation, postoperative patients, osteochondrosis, others) and subjected to nucleolysis with ozone and to periradicular infiltration with steroids and local anaesthesia. The success of treatment was assessed by means of a visual analog pain scale (VAS) and the Oswestry Disability Index (ODI).

A significant reduction in the VAS was registered after 2 and 6 months (from 8.6 to 5.4 and 6.0; p < 0.001) in all patient groups; an excellent therapy response (VAS below 3.0) was achieved by about a third of the patients. A significant improvement in ODI was registered in all patients (46 to 31; p < 0.001), most pronounced in the herniation group (25.5, p = 0.015). Patients below 50 years had significantly better values in the VAS and ODI score 6 months after treatment. Final VAS and ODI scores for patients with a single diseased segment were 4.2 and 28.0, in two affected segments 6.5 and 32 and in three segments 6.7 and 38.5 (p < 0.001 and p = 0.051).

ONL with periradicular steroid therapy might exert a functional and sustained analgesic effect in patients with degenerative changes in the lumbar spine not responding to conservative therapy and was most effective below 50 years with disc herniation in one segment.

PMID: 18483728 [PubMed - indexed for MEDLINE]

Knee-joint disorders treated by oxygen-ozone therapy.
Riva Sanseverino, E.
Ordinario di Fisiologia umana nell'Universit´┐Ż di Bologna, Italy
Europa Medicophysica 25(3): 163-170, 1989.
Journal written in English


In patients affected by different forms of knee disorders, the effects of oxygen-ozone mixture (the so-called medical ozone) locally injected were studied. 20 ml of the mixture were introduced into the knee with a concentration of 10mg of ozone per ml of oxygen. When necessary, the same concentration of the mixture was also peri-articularly and subcutaneously administered. 156 patients were treated and divided into 3 groups: carriers of post-traumatic knee disorders, gonarthrosis without marked bone deformities and gonatrhrosis with marked bone deformities. In the first two groups optimal results were obtained while for the third other therapies were then added to stop the advancing degenerative process. In conclusion, knee-joint disorders can very successfully be treated by means of oxygen-ozone mixture locally administered.

AJNR Am J Neuroradiol. 2005 May;26(5):996-1000.

Intraforaminal O(2)-O(3) versus periradicular steroidal infiltrations in lower back pain: randomized controlled study.
Bonetti M, Fontana A, Cotticelli B, Volta GD, Guindani M, Leonardi M.

Department of Neuroradiology and Division of Neurology, Istituto Clinico Cittá di Brescia, Italy.


Reports about steroids and oxygen-ozone therapy to treat lower back pain have been increasing. The purpose of our study was to compare the clinical outcomes in patients treated with infiltrations of O(2)-O(3) gas or steroids at short-, medium-, and long-term follow-up.

A total of 306 patients (166 with primarily disk disease, 140 with nondisk vertebral disease) with acute or chronic low back and sciatic nerve pain received a CT-guided intraforaminal infiltration of an O(2)-O(3) gas mixture or an periradicular infiltration of steroids. Neurologists unaware of the type of treatment assessed the patients.

At 1-week follow-up, most patients had a complete remission of pain, regardless of the treatment. At 6-month follow-up, differences in favor of O(2)-O(3) treatment were significant in patients with disk disease (P = .0021) but not in those without disk disease (P = .0992). Clinical outcomes were poor in 13 (15.1%) of 86 patients receiving O(2)-O(3) infiltration and in 18 (22.5%) of 80 patients receiving steroid injection (P = .2226). Among patients without disk disease, six (8.6%) of 70 patients receiving O(2)-O(3) infiltration but 21.4% of the patients receiving steroid injections had poor outcomes (P = .0332).

Oxygen-ozone treatment was highly effective in relieving acute and chronic lower back pain and sciatica. The gas mixture can be administered as a first treatment to replace epidural steroids.

Comment in Ozone therapy and lower back pain. [AJNR Am J Neuroradiol. 2006] Therapeutic periradicular injections: it's a gas! [AJNR Am J Neuroradiol. 2005] PMID:15891150 [PubMed - indexed for MEDLINE]

Spine (Phila Pa 1976). 2009 Jun 1;34(13):1337-44. doi: 10.1097/BRS.0b013e3181a3c18d.
Intramuscular oxygen-ozone therapy in the treatment of acute back pain with lumbar disc herniation: a multicenter, randomized, double-blind, clinical trial of active and simulated lumbar paravertebral injection.
Paoloni M, Di Sante L, Cacchio A, Apuzzo D, Marotta S, Razzano M, Franzini M, Santilli V.

Physical Medicine and Rehabilitation Unit, Azienda Policlinico Umberto I, Rome, Italy.


Multicenter randomized, double-blind, simulated therapy-controlled trial in a cohort of patients with acute low back pain (LBP) due to lumbar disc herniation (LDH).

To assess the benefit of intramuscular-paravertebral injections of an oxygen-ozone (O2O3) mixture.

Recent findings have shown that O2O3 therapy can be used to treat LDH that fails to respond to conservative management. However, these findings are based on intradiscal/intraforaminal O2O3 injection, whereas intramuscular-paravertebral injection is the technique used most in clinical practice in Italy and other Western countries.

Sixty patients suffering from acute LBP caused by LDH was randomized to an intramuscular O2O3 or control group. Patients were observed up to assess pain intensity, LBP-related disability, and drug intake (15 [V2] and 30 [V3] days after treatment started, and 2 weeks [V4], and 3 [V5] and 6 [V6] months after treatment ended).

A significant difference between the 2 groups in the percentage of cases who had become pain-free (61% vs. 33%, P < 0.05) was observed at V6. Patients who received O2O3 had a lower mean pain score than patients who received simulated therapy throughout the observation period. A significant improvement was observed in LBP-related disability in the study group patients when compared with the control group patients. Active O2O3 therapy was followed by a significantly lower number of days on nonsteroidal anti-inflammatory drugs at V2 and V3 and by a lower number of days at V4. No adverse events were reported.

Treatment of LBP and sciatica is a major concern. Although the natural history of acute LBP is often self-limiting, conservative therapies are not always effective; in such cases, O2O3 intramuscular lumbar paravertebral injections, which are minimally invasive, seem to safely and effectively relieve pain, as well as reduce both disability and the intake of analgesic drugs.

PMID: 19478653 [PubMed - indexed for MEDLINE] Acta Neurochir Suppl. 2011;108:137-42. doi: 10.1007/978-3-211-99370-5_21.

Oxygen-ozone therapy for degenerative spine disease in the elderly: a prospective study.
Bonetti M, Fontana A, Martinelli F, Andreula C.

Servizio di Neuroradiologia, Istituto Clinico Cittá di Brescia, Via Gualla 15, 25123, Brescia, Italy.

We describe our experience of oxygen-ozone therapy to treat degenerative spine disease in the elderly. From April 2004 to March 2008 we selected 129 patients with CT and/or MR evidence of spondyloarthrosis and disc degeneration of the lumbar spine. All patients enrolled in the study had contraindications to the administration of commonly used analgesic and anti-inflammatory drugs.Oxygen-ozone therapy was given by CT-guided intraforaminal injection as the first treatment followed by 4 weekly paralumbar infiltrations on an outpatient basis. The full treatment lasted a month. Clinical outcome was assessed 3 months and 1 year after treatment. The good results obtained indicate that oxygen-ozone therapy is an ideal treatment with no side-effects in elderly patients with degenerative spine disease.

PMID: 21107950 [PubMed - indexed for MEDLINE]

J Vasc Interv Radiol. 2010 Apr;21(4):534-48. doi: 10.1016/j.jvir.2009.12.393. Epub 2010 Feb 25.
A metaanalysis of the effectiveness and safety of ozone treatments for herniated lumbar discs.
Steppan J, Meaders T, Muto M, Murphy KJ.

ActiveO, Salt Lake City, Utah, USA.


To determine statistically significant effects of oxygen/ozone treatment of herniated discs with respect to pain, function, and complication rate.

Random-effects metaanalyses were used to estimate outcomes for oxygen/ozone treatment of herniated discs. A literature search provided relevant studies that were weighted by a study quality score. Separate metaanalyses were performed for visual analog scale (VAS), Oswestry Disability Index (ODI), and modified MacNab outcome scales, as well as for complication rate. Institutional review board approval was not required for this retrospective analysis.

Twelve studies were included in the metaanalyses. The inclusion/exclusion criteria, patient demographics, clinical trial rankings, treatment procedures, outcome measures, and complications are summarized. Metaanalyses were performed on the oxygen/ozone treatment results for almost 8,000 patients from multiple centers. The mean improvement was 3.9 for VAS and 25.7 for ODI. The likelihood of showing improvement on the modified MacNab scale was 79.7%. The means for the VAS and ODI outcomes are well above the minimum clinically important difference and the minimum (significant) detectable change. The likelihood of complications was 0.064%.

Oxygen/ozone treatment of herniated discs is an effective and extremely safe procedure. The estimated improvement in pain and function is impressive in view of the broad inclusion criteria, which included patients ranging in age from 13 to 94 years with all types of disc herniations. Pain and function outcomes are similar to the outcomes for lumbar discs treated with surgical discectomy, but the complication rate is much lower (<0.1%) and the recovery time is significantly shorter.

Copyright 2010 SIR. Published by Elsevier Inc. All rights reserved.
PMID: 20188591 [PubMed - indexed for MEDLINE]